Services

EDS Services List (Rev. 01/11/2017)

EDS Test Request Form (Rev. 01/11/2017)

EDS Test Descriptions and Guidelines

Equine Protozoal Myeloencephalitis (EPM):

Sarcocystis neurona SAG 2/3/4 ELISA

This assay was developed in the lab of Dr. Dan Howe at the University of Kentucky’s Gluck Equine Research Center and is available exclusively at EDS. An endpoint titer provides quantified results based on the IgG response to three S. neurona specific antigens. These antigens have been shown to correlate well with the standard western blot, clinical diagnosis and necropsy. Paired serum and cerebrospinal fluid (CSF) are the recommended samples. Serum:CSF titer ratios are highly predictive for distinguishing EPM from other neurologic diseases.

Sarcocystis neurona western blot

The S. neurona standard western blot performed at EDS is based on the method developed and validated at the Gluck Equine Research Center at the University of Kentucky (Dr. David Granstrom 1993). It detects the IgG antibody response to specific S. neurona antigens resulting from exposure to and infection by the parasite. Results are semi-quantified (positive, low positive, weak positive, negative) based on intensity and the specific antigen banding patterns as compared to control sera or CSF.

Sarcocystis neurona PCR

The PCR test detects S.neurona DNA and thus the actual presence of the parasite. This assay is performed on CSF and is best used in conjunction with the western blot or ELISA test.

Neospora hughesi ELISA

The Neospora hughesi ELISA was developed in the lab of Dr. Dan Howe at the University of Kentucky's Gluck Equine Research center. Serum and/or CSF samples can be tested for an IgG response to the N. hughesi parasite. The ELISA generates an endpoint titer and paired serum and CSF samples can be used to calculate a Serum:CSF titer ratio which is predictive for an EPM diagnosis.

S. neurona specific index

The specific index is performed on paired serum and CSF samples in conjunction with the S. neurona SAG 2/3/4 ELISA. This test can help discern an intrathecal IgG response from contamination by utilizing a ratio of serum and CSF albumin and IgG levels. It is very helpful in the interpretation of results when the CSF has been contaminated with blood or when the Serum:CSF ratio is equal to 100.

Streptococcus equi (strangles):

Streptococcus equi real-time PCR

The PCR test detects S. equi bacterial DNA and is used primarily to identify asymptomatic carriers. EDS lab's PCR method employs two separate gene targets in the PCR reaction for improved specificity and sensitivity of test results. This PCR is an effective screening tool for systematically evaluating and managing resident and new horses on a property to control and prevent potential outbreaks. Nasal washes, nasal swabs or guttural pouch washes are appropriate samples for the S. equi PCR. Back up culture is also available.

Streptococcus equi SEM ELISA

The SeM ELISA test was developed at the Gluck Equine Research Center at the University of Kentucky (Dr. John Timoney) and assesses the IgG endpoint titer against the highly immunogenic S. equi specific M protein. It is helpful for making vaccination decisions on horses with existing titers, identifying horses at risk of complications due to elevated titers, or for horses with aberrant abscesses (bastard strangles). The SEM ELISA should not be used to determine the infection status of horses with clinical symptoms of strangles.

Equine Herpesvirus (EHV):

EHV-1 real-time PCR

This PCR test detects EHV-1 strains associated with respiratory disease, abortion, and will also distinguish if the ORF30 (DNA polymerase gene) mutation is present. The presence of this mutation has a high correlation with the neurological form of this disease, equine herpes myeloencephalopathy (EHM). EDS recommends submission of both whole blood (EDTA) and a nasal swab.

EHV-4 real-time PCR

This PCR test detects EHV-4 strains which are generally associated with respiratory disease and a nasal swab is the recommended sample.

Rhodococcus equi real-time PCR:

The PCR test identifies strains of R. equi by detecting the virulence plasmid gene vapA. The vapA gene is highly associated with the ability to cause clinical disease and pneumonia in foals. A trans-tracheal wash is the recommended specimen for R. equi PCR, however, a nasal swab or fecal sample can also be tested. Back up culture is also available.

Salmonella spp. real-time PCR:

The PCR test detects DNA from pathogenic species of Salmonella and can be used as a bio-surveillance tool for the identification of asymptomatic/shedding horses. This assay is also useful for the monitoring of in-patients in a hospital setting. Feces or a fecal swab is the recommended specimen, EDS lab is also able to test environmental swabs for Salmonella species. Back up culture is also available.

Equine Influenza Virus (EIV) real-time PCR:

This test detects the H3N8 and H7N7 (New York) strains of EIV and a nasal swab is the recommended sample. EIV PCR is included in the our basic respiratory panel.

Equine Rhinitis Virus A and B real-time PCR:

Equine Rhinitis Virus type A and type B are both detected by this test. Urine is the recommended sample for this respiratory pathogen as shedding by the urinary tract has a longer window than by nasal secretions, a nasal swab or wash can also be tested. This test is part of our expanded respiratory panel.

Equine Arteritis Virus (EAV) real-time PCR:

EAV RNA is detected by this PCR test. Arteritis virus is associated with respiratory disease in horses but more significantly may be associated with abortions. Appropriate specimens for submission include nasal swabs or washes, semen, whole blood (EDTA), or ocular swabs. PCR is not an accepted test method for export. This test is part of our expanded respiratory panel.

Lawsonia intracellularis ELISA:

This assay was developed by Dr. Allen Page in the lab of Dr. Dave Horohov at the University of Kentucky's Gluck Equine Research Center. It is available exclusively at EDS. To support diagnosis of equine proliferative enteropathy (EPE) the presence of serum antibodies, with concurrent hypoalbuminemia or hypoproteinemia, indicates a recent or current infection.

West Nile Virus (WNV) IgM ELISA:

The IgM ELISA test is used to detect recent exposure to the west nile virus. Presence of IgM antibodies against WNV are diagnostic of current or recent infection. This assay is included in our neurologic panels.

Equine Infectious Anemia Virus (EIAV):

EIA AGID and EIA ELISA

EDS is a federally accredited lab for this regulated testing and offers both approved test formats: AGID (Coggins) and ELISA. Please submit a fully completed and signed form with each sample.

Panels and Profiles:

Several combinations of different, but complementary, tests are also available. Please refer to the service list for a complete listing.

 

Test Request Form (Rev. 01/11/2017)

 

Last Updated on Tuesday, 02 May 2017 00:02
 
Services

EDS Services List (Rev. 01/11/2017)

EDS Test Request Form (Rev. 01/11/2017)

EDS Test Descriptions and Guidelines

Equine Protozoal Myeloencephalitis (EPM):

Sarcocystis neurona SAG 2/3/4 ELISA

This assay was developed in the lab of Dr. Dan Howe at the University of Kentucky’s Gluck Equine Research Center and is available exclusively at EDS. An endpoint titer provides quantified results based on the IgG response to three S. neurona specific antigens. These antigens have been shown to correlate well with the standard western blot, clinical diagnosis and necropsy. Paired serum and cerebrospinal fluid (CSF) are the recommended samples. Serum:CSF titer ratios are highly predictive for distinguishing EPM from other neurologic diseases.

Sarcocystis neurona western blot

The S. neurona standard western blot performed at EDS is based on the method developed and validated at the Gluck Equine Research Center at the University of Kentucky (Dr. David Granstrom 1993). It detects the IgG antibody response to specific S. neurona antigens resulting from exposure to and infection by the parasite. Results are semi-quantified (positive, low positive, weak positive, negative) based on intensity and the specific antigen banding patterns as compared to control sera or CSF.

Sarcocystis neurona PCR

The PCR test detects S.neurona DNA and thus the actual presence of the parasite. This assay is performed on CSF and is best used in conjunction with the western blot or ELISA test.

Neospora hughesi ELISA

The Neospora hughesi ELISA was developed in the lab of Dr. Dan Howe at the University of Kentucky's Gluck Equine Research center. Serum and/or CSF samples can be tested for an IgG response to the N. hughesi parasite. The ELISA generates an endpoint titer and paired serum and CSF samples can be used to calculate a Serum:CSF titer ratio which is predictive for an EPM diagnosis.

S. neurona specific index

The specific index is performed on paired serum and CSF samples in conjunction with the S. neurona SAG 2/3/4 ELISA. This test can help discern an intrathecal IgG response from contamination by utilizing a ratio of serum and CSF albumin and IgG levels. It is very helpful in the interpretation of results when the CSF has been contaminated with blood or when the Serum:CSF ratio is equal to 100.

Streptococcus equi (strangles):

Streptococcus equi real-time PCR

The PCR test detects S. equi bacterial DNA and is used primarily to identify asymptomatic carriers. EDS lab's PCR method employs two separate gene targets in the PCR reaction for improved specificity and sensitivity of test results. This PCR is an effective screening tool for systematically evaluating and managing resident and new horses on a property to control and prevent potential outbreaks. Nasal washes, nasal swabs or guttural pouch washes are appropriate samples for the S. equi PCR. Back up culture is also available.

Streptococcus equi SEM ELISA

The SeM ELISA test was developed at the Gluck Equine Research Center at the University of Kentucky (Dr. John Timoney) and assesses the IgG endpoint titer against the highly immunogenic S. equi specific M protein. It is helpful for making vaccination decisions on horses with existing titers, identifying horses at risk of complications due to elevated titers, or for horses with aberrant abscesses (bastard strangles). The SEM ELISA should not be used to determine the infection status of horses with clinical symptoms of strangles.

Equine Herpesvirus (EHV):

EHV-1 real-time PCR

This PCR test detects EHV-1 strains associated with respiratory disease, abortion, and will also distinguish if the ORF30 (DNA polymerase gene) mutation is present. The presence of this mutation has a high correlation with the neurological form of this disease, equine herpes myeloencephalopathy (EHM). EDS recommends submission of both whole blood (EDTA) and a nasal swab.

EHV-4 real-time PCR

This PCR test detects EHV-4 strains which are generally associated with respiratory disease and a nasal swab is the recommended sample.

Rhodococcus equi real-time PCR:

The PCR test identifies strains of R. equi by detecting the virulence plasmid gene vapA. The vapA gene is highly associated with the ability to cause clinical disease and pneumonia in foals. A trans-tracheal wash is the recommended specimen for R. equi PCR, however, a nasal swab or fecal sample can also be tested. Back up culture is also available.

Salmonella spp. real-time PCR:

The PCR test detects DNA from pathogenic species of Salmonella and can be used as a bio-surveillance tool for the identification of asymptomatic/shedding horses. This assay is also useful for the monitoring of in-patients in a hospital setting. Feces or a fecal swab is the recommended specimen, EDS lab is also able to test environmental swabs for Salmonella species. Back up culture is also available.

Equine Influenza Virus (EIV) real-time PCR:

This test detects the H3N8 and H7N7 (New York) strains of EIV and a nasal swab is the recommended sample. EIV PCR is included in the our basic respiratory panel.

Equine Rhinitis Virus A and B real-time PCR:

Equine Rhinitis Virus type A and type B are both detected by this test. Urine is the recommended sample for this respiratory pathogen as shedding by the urinary tract has a longer window than by nasal secretions, a nasal swab or wash can also be tested. This test is part of our expanded respiratory panel.

Equine Arteritis Virus (EAV) real-time PCR:

EAV RNA is detected by this PCR test. Arteritis virus is associated with respiratory disease in horses but more significantly may be associated with abortions. Appropriate specimens for submission include nasal swabs or washes, semen, whole blood (EDTA), or ocular swabs. PCR is not an accepted test method for export. This test is part of our expanded respiratory panel.

Lawsonia intracellularis ELISA:

This assay was developed by Dr. Allen Page in the lab of Dr. Dave Horohov at the University of Kentucky's Gluck Equine Research Center. It is available exclusively at EDS. To support diagnosis of equine proliferative enteropathy (EPE) the presence of serum antibodies, with concurrent hypoalbuminemia or hypoproteinemia, indicates a recent or current infection.

West Nile Virus (WNV) IgM ELISA:

The IgM ELISA test is used to detect recent exposure to the west nile virus. Presence of IgM antibodies against WNV are diagnostic of current or recent infection. This assay is included in our neurologic panels.

Equine Infectious Anemia Virus (EIAV):

EIA AGID and EIA ELISA

EDS is a federally accredited lab for this regulated testing and offers both approved test formats: AGID (Coggins) and ELISA. Please submit a fully completed and signed form with each sample.

Panels and Profiles:

Several combinations of different, but complementary, tests are also available. Please refer to the service list for a complete listing.

 

Test Request Form (Rev. 01/11/2017)

 

Last Updated on Tuesday, 02 May 2017 00:02