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EDS Test Request Form (Rev. 01/01/2014)

EDS Test Descriptions and Guidelines

Equine Protozoal Myeloencephalitis (EPM):

Sarcocystis neurona SAG 2/3/4 Titer (Sarcocystis neurona ELISA):
This assay was developed in the lab of Dr. Dan Howe at the University of Kentucky’s Gluck Equine Research Center and is available exclusively at EDS. An endpoint titer provides quantified results based on the IgG response to three S. neurona specific antigens-SAG 2, 3 & 4. These antigens have been shown to correlate well with the standard western blot, clinical diagnosis and necropsy. Paired serum and cerebrospinal fluid (CSF) are the recommended samples. Serum:CSF titer ratios are highly predictive for distinguishing EPM from other neurologic diseases.

Sarcocystis neurona western blot

The S. neurona standard western blot performed at EDS is based on the method developed and validated at the Gluck Equine Research Center at the University of Kentucky (Dr. David Granstrom 1993). It detects the IgG antibody response to specific S. neurona antigens resulting from exposure to and infection by the parasite. Results are semi-quantified (positive, low positive, weak positive, negative) based on intensity and the specific antigen banding patterns as compared to control sera or CSF.

Sarcocystis neurona PCR

The PCR test detects S.neurona DNA and thus the actual presence of the parasite. This assay is performed on CSF and is best used in conjunction with the western blot or ELISA test.

Neospora hughesi ELISA

This assay was developed in the lab of Dr. Dan Howe at the University of Kentucky Gluck Equine Research Center. Serum and/or CSF can be tested and an endpoint titer is generated. Serum:CSF titer ratios are predictive for distinguishing EPM from other neurologic diseases.

CSF Index and S. neurona specific Index

The CSF index is performed on paired serum and CSF samples. This test can help discern an intrathecal IgG response from contamination by utilizing a ratio of serum and CSF albumin and IgG levels. When used in conjunction with the S. neurona Titers,  a specific antibody indexes or C-values can be calculated.  The specific index can help to interpret titer results that are borderline or from CSF samples that have blood contamination.

 

Streptococcus equi (strangles):

Streptococcus equi PCR

The PCR test detects S. equi bacterial DNA and is used primarily to identify asymptomatic carriers. It is an effective screening tool for systematically evaluating and managing resident and new horses on a property to control and prevent potential outbreaks. Nasal washes, nasal swabs or guttural pouch washes are appropriate samples for the S. equi PCR. Back up culture is also available.

Streptococcus equi SEM ELISA

The SeM ELISA test was developed at the Gluck Equine Research Center at the University of Kentucky (Dr. John Timoney) and assesses the IgG endpoint titer against the highly immunogenic S. equi specific M protein. It is helpful for making vaccination decisions on horses with existing titers, identifying horses at risk of complications due to elevated titers, or for horses with aberrant abscesses (bastard strangles).

 

West Nile Virus (WNV) IgM ELISA:

The IgM ELISA test is used to detect recent exposure to the virus. This assay is included in our neurologic panels.

 

Equine Herpesvirus (EHV) PCR:

EHV1

This PCR test detects EHV-1 strains and will also distinguish if the ORF30 (DNA polymerase gene) mutation is present. The presence of this mutation has a high correlation with the neurological form of this disease. EDS recommends submission of both whole blood and a nasal swab for a neurologic case and nasal swab only for a respiratory case.

EHV4

This PCR test detects EHV-4 strains which are generally associated with respiratory disease and a nasal swab is the recommended sample.

 

 

Equine Infectious Anemia Virus (EIAV):

EIA AGID

EIA c-ELISA

EDS is a federally accredited lab for this regulated testing and offers both approved test formats: AGID (Coggins) and ELISA. Please submit a fully completed and signed form with each sample.

Equine Rhinitis Virus Types A and B (ERAV/ERBV) PCR

Equine rhinitis virus has been an under recognized cause of respiratory disease until recently. ERV can cause mild to severe primary respiratory disease and secondarily, inflammatory airway disease and airway hyper-responsiveness.  In addition to nasal spreading of infectious viral particles, highly viable ERAV is present in urine for a prolonged period of time. Urine, nasal washes or pharyngeal swabs are acceptable specimens for this assay.

Rhodococcus equi (foal pneumonia) PCR

The PCR test identifies strains of R. equi by detecting the virulence plasmid gene vapA. The vapA gene is highly associated with the ability to cause clinical disease. Back up culture is also available.

 

Salmonella spp. PCR:

The PCR test detects DNA from pathogenic species of Salmonella and can be used as a bio-surveillance tool for the identification of asymptomatic/shedding horses. This assay is also useful for the monitoring of in-patients in a hospital setting. Back up culture is also available.

 

Equine Influenza Virus (EIV) PCR:

This test detects the H3N8 and H7N7 (New York) strains of EIV and a nasal swab is the recommended sample.

 

Panels and Profiles:

Several combinations of different, but complementary, tests are also available. Please refer to the service list for a complete listing.

EDS Test Request Form (Rev. 01/01/2014)

 

Last Updated on Friday, 17 January 2014 01:02